The visualization of single proteins through electron microscopy and using metal nanoparticles is mostly acchieved through immuno-gold nanoparticle precipitation, however there are technological limitation with this technology.
In this project, you will explore genetically encoded reporters for the visualization of single proteins in a cell-wide manner using electron microscopy and programmable nucleases. The project aims to create stable lines coding the genetically encoded reporters onto proteins of therapeutic interest and within human cell systems, including human iPS cells, and immune effector cells.
In our project, you will learn how to optimize differentiation protocols aided by genome engineering. You will create cellular master stocks of engineered cells, that could be used for mechanistic dissecting through EM visualization in close collaboration with partners. You will be hosted at the EMBL-VU Institute for Genome Editing Technologies. You will be supervised by Dr. Jonathan Arias (Group Leader), expert in genome engineering and iPS cell platforms. He will guide you to create scientific articles and patents with commercial potential. Learn more about us at https://www.gmc.vu.lt/en/group-of-cell-therapeutics.