Monoamine oxidases A and B (MAO-A and MAO-B) are pivotal in the metabolism of neurotransmitters, thus playing a critical role in regulating their levels within the human body. Consequently, inhibitors targeting these enzymes hold significant therapeutic potential for treating conditions such as depression, Parkinson's disease, and other neurological disorders. Therefore, the development of efficient methods for rapidly detecting inhibitors of MAO-A and MAO-B is paramount for advancements in modern medicine. This PhD research aims to identify novel reversible inhibitors that selectively target either MAO-A or MAO-B enzymes through the utilization of electrochemical enzymatic biosensors. Established dyes and organic compounds will be employed in this pursuit. The inhibitory properties of the selected compounds will be systematically compared with those of well-known drugs. Furthermore, theoretical simulations will be conducted to explore the active centers of these enzymes in conjunction with potential inhibitors, aiming to elucidate structural trends among the inhibitors.