Bacterial infections are one of the most common causes of death, especially in developing countries. The diagnosis and differentiation of these infections from viral diseases using classical methods (culture and/or PCR) is relatively slow and expensive. Therefore, new research methods, devices and solutions are needed to create tools for the simple and reliable detection of bacteria and at the same time for the diagnosis of diseases caused by bacteria. The aim of this PhD thesis is to develop biosensors for the detection of bacterial markers that could be used for a fast and efficient diagnosis of bacterial diseases. During the PhD studies, the following tasks will be carried out: i) search for bacterial biomarkers; ii) development of methods to detect and determine these biomarkers using spectrophotometric and/or bioelectrochemical analysis methods; iii) adaptation of the methods to determine bacterial biomarkers; iv) detection of biomarkers in real samples to identify bacterial infections; v) increasing the maturity of the developed technology with a view to possible application. After successful development of the technology, the PhD student would be offered the opportunity to participate in the commercialization of the technology.