Short Description: ÄKTA Pure Micro is a compact and highly precise liquid chromatography system designed for low-volume, high-sensitivity purification of proteins and other biomolecules. The system minimizes sample loss and ensures excellent reproducibility, making it ideal for working with limited or valuable samples.

Produser: Cytiva

Equipment information:

Functions:

• Automated low-volume purification of biomolecules such as proteins, peptides, and nucleic acids.
• Highly precise control of flow rate, pressure, and buffer gradients in micro-chromatography mode.
• Real-time detection using high-sensitivity UV and other integrated detectors.

Capabilities:

• Designed to work with very small sample volumes, minimizing sample loss.
• Supports multiple chromatography techniques: affinity, ion exchange, hydrophobic interaction (HIC), and size-exclusion chromatography (SEC).
• Modular configuration allows the system to be adapted to different workflows, including multi-step purification.
• Automated fraction collection based on UV signal, time, or volume.
• The system is housed in a chromatographic refrigerator (+5 °C), allowing work with samples that are sensitive to higher temperatures.
• Compatible with UNICORN software for method development, automation, and data analysis.

Purpose:

• Intended for research applications where precise purification of small quantities of proteins is required.
• Ideal for valuable or limited-availability samples (e.g., recombinant proteins, biomarkers, antibody fragments).
• Designed for micro-scale chromatography workflows that require high sensitivity and low flow rates.

Applications:

• Micro-scale purification of proteins and peptides.
• Preparation of analytical or preparative micro-fractions for structural, biochemical, or functional studies.
• Purification of recombinant proteins, antibody fragments, and biological markers.
• Method optimization prior to scale-up to larger chromatography systems.

Working Principle:

• The system pumps the sample through a chromatography column packed with resin, to which biomolecules bind based on their chemical or physical properties (such as affinity, charge, size, or hydrophobicity). By adjusting buffer composition or ionic strength, the target molecule is released and eluted. Integrated detectors monitor the elution profile in real time, generating a chromatogram that enables precise identification and collection of desired fractions.

Technical Specification:

Software: Unicorn 7.6

VU ID: 8151244
Location: BVI 116 
Core Facility: Bioengineering

Equipment is operated by Personnel only: Yes
It is allowed to work with the equipment independently after familiarization with the work process on site: No
Only those with the appropriate certificate/certificate are allowed to work with the equipment: Yes
There is an opportunity to participate in equipment usage training: No

Contact Scientist (Leading Scientist):
Dr. Arūnas Šilanskas,
El.paštas: 
Tel.: +370 600 09405

Information for the User:

• AKTA Pure Operating Instructions (.pdf)