Name and Surname of the Supervisor: Jonathan Arias
Institution (Company), Department: VU EMBL partnership institute
Preliminary Topic: Optimization of gene editing on human naive stem cells - cells with higher pluripotency
Short Description of the Internship Work: Naïve pluripotent stem cells (nPSCs) approximate the pre‑implantation epiblast, whereas “primed” PSCs resemble the post‑implantation epiblast. A cytokine‑independent “ground state” can be stabilized by dual inhibition of MEK/ERK and GSK3 (“2i”), establishing the canonical biochemical reference for naïve pluripotency. In humans, short‑term expression of NANOG/KLF2 coupled with small‑molecule conditions can reset primed PSCs to a naïve‑like state with transcriptomic and epigenetic features closer enhanced pluripotency.
In this project you will implement induced naïve pluripotent stem cells (inPSCs) cultures and optimize gene editing on them.
For more info, contact the team leader.
Qualification Requirements (Degree): BSc MSc MSc completed
Application Deadline: 2025-12-01
Additional Comments: For applicaiton, please share a 1 paragraph with your authentic interests.
Comment about any previous experience on cell culture or molecular biology.
Attach course transcripts.