Name and Surname of the Supervisor: Marijonas Tutkus
Institution (Company), Department: Institute of Biotechnology, LSC, VU
Preliminary Topic: Live-Cell Multi-Locus Chromosome Labelling and Tracking
Short Description of the Internship Work: Are you interested in cutting-edge research at the intersection of biophysics, molecular biology, and advanced microscopy? Join Dr. Marijonas Tutkus's laboratory (more info here: https://tutkuslab.github.io/) for a professional practice opportunity where you'll contribute to groundbreaking research in chromosome dynamics visualization!
About the Project
This professional practice is part of the innovative project, which aims to study live-cell nanoscale chromosome dynamics, break repair, and rearrangements across generations. You will be involved in developing novel techniques for live-cell multi-locus chromosome labelling and tracking, specifically focusing on:
1. Development and optimization of a novel method for probing live-cell multi-locus chromosome dynamics using fluorescently labelled Cas complexes
2. Working with both regular and novel "gentle PAINT" labelling approaches utilizing dCas12a protein as a probe
3. Implementation of chromosome barcoding techniques to identify different loci simultaneously
4. Testing and optimizing these methods in fission yeast as a model system.
What You'll Gain
a. Hands-on experience with state-of-the-art super-resolution microscopy and single-molecule tracking
b. Training in advanced molecular labelling techniques for live-cell imaging
c. Exposure to multidisciplinary research at the interface of biophysics, optics, and molecular biology
d. Opportunity to contribute to research with significant implications for understanding fundamental cell biology processes and gene editing therapies
About the Laboratory
The research will be conducted at Dr. Marijonas Tutkus's lab located at the Department of Protein-DNA Interactions, Institute of Biotechnology, Life Sciences Center of Vilnius University. Our team is developing groundbreaking tools to visualize chromosome dynamics in living cells with unprecedented precision and detail.
How to Apply
Interested candidates should send their CV, a brief motivation letter explaining their interest in the project, and their weekly availability to [].
Join us in developing innovative methods that will enhance our understanding of fundamental cell biology processes and contribute to establishing the long-term safety of novel gene editing therapies!
Qualification Requirements (Degree): BSc, MSc, MSc completed
Application Deadline: 2025-06-01
Additional Comments: Requirements for a candidate:
General knowledge of microscopy principles and techniques
Laboratory skills with experience in biochemistry and molecular biology methods
Skills in working with standard data analysis programs
Basic programming knowledge
Ability to devote 1-2 full days per week to laboratory work
Strong interest in cutting-edge bioimaging and chromosome biology