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Enzyme functional analysis is crucial for evaluating enzyme performance. However, even well-known enzymes, such as laccase, face chalenges with substrates and methods for activity assays. The review examines both commonly and less-utilized compounds, as well as their associated techniques, methods, and approaches. The review highlights potential strategies to overcome identified gaps. |
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Wood sugars enabled successful CA production by yeast, bacteria, and fungi. Bacterial strains highlighting the potential for CA production. Highest CA yields: 5.7 g/L bacterial, 36.8 g/L yeast, 67.7 g/L fungi fermentation. The application of ex-ante LCA resulted in 4–6 kgCO2eq/kg citric acid. |
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J. Babinskas, J. Krutkevičius, L. Lukić Bilela, R. Bešta-Gajević, I. Matijošytė. Potential of subterranean microbes: High‐throughput screening for industrially relevant enzymatic activities in Dinaric caves. Microbiologyopen 2025, 14. doi.org/10.1002/mbo3.70146
L. Leišytė, I.N. Braje, S. Almog, S. Baysan, T. Carvalho, D. Daunoraitė, S. Diogo, P. Papaioannou, A. Farmaki, S. Feldman, R. Külcür, I. Matijošytė, S. Pragauskaitė, V. Rangelova, D. Šatkovskienė. Early career researchers as stakeholders in university decision‐making in Europe: Comparative perspectives. Soc. Incl. 2025, 14, 9683. doi.org/10.17645/si.9683
J. Babinskas, I. Matijošytė. Laccase functional analysis: substrates, activity assays, challenges, and prospects. ChemBioChem 2025, 26, e202400939. doi.org/10.1002/cbic.202400939
D. Daunoraitė, N.R. Dias de Souza, J. Babinskas, F. Cherubini, L. Vares, I. Matijošytė. Unlocking nature's sweet secret for citric acid production from wood sugars: Evaluation of microbial strains and environmental impacts. J. Clean. Prod. 2024, 467, 142914. doi.org/10.1016/j.jclepro.2024.142914
J. Babinskas, J. Sabotič, I. Matijošytė. Synthesis and application of a phenazine class substrate for high-throughput screening of laccase activity. Appl. Microbiol. Biotechnol. 2024, 108, 1. doi.org/10.1007/s00253-023-12958-7
L.L. Bilela, I. Matijošytė, J. Krutkevičius, D.A.M. Alexandrino, I. Safarik, J. Burlakovs, S.P. Gaudêncio, M.F. Carvalho. Impact of per- and polyfluorinated alkyl substances (PFAS) on the marine environment: Raising awareness, challenges, legislation, and mitigation approaches under the One Health concept. Mar. Pollut. Bull. 2023, 194 (A), 115309. doi.org/10.1016/j.marpolbul.2023.115309
A. Rotter, M. Barbier, F. Bertoni, A.M. Bones, M.L. Cancela, J. Carlsson, M.F. Carvalho, M. Cegłowska, J. Chirivella-Martorell, M.C. Dalay, M. Cueto, T. Dailianis, I. Deniz, A.R. Díaz-Marrero, D, Drakulovic, A. Dubnika, C. Edwards, H. Einarsson, A. Erdogan, O.T. Eroldogan, D. Ezra, S. Fazi, R.J. FitzGerald, L.M. Gargan, S.P. Gaudêncio, M.G. Udovic, N.I. DeNardis, R. Jónsdóttir, M. Kataržyte, K. Klun, J. Kotta, L. Ktari, Z. Ljubešic, L.L. Bilela, M. Mandalakis, A. Massa-Gallucci, I. Matijošytė, H. Mazur-Marzec, M. Mehiri, S.L. Nielsen, L. Novoveská, D. Overlinge, G. Perale, P. Ramasamy, C. Rebours, T. Reinsch, F. Reyes, B. Rinkevich, J. Robbens, E. Röttinger, V. Rudovica, J. Sabotic, I. Safarik, S. Talve, D. Tasdemir, X.T. Schneider, O.P. Thomas, A. Torunska-Sitarz, G.C. Varese, M.I. Vasquez. The essentials of marine biotechnology. Front. Mar. Sci. 2021, 8, 629629. doi.org/10.3389/fmars.2021.629629
M. Baliukynas, A. Veteikytė, V. Kairys, I. Matijošytė. The hydrolysis of indoxyl acetate: A versatile reaction to assay carbonic anhydrase activity by high-throughput screening. Enzyme Microb. Technol. 2020, 136, 109584. doi.org/10.1016/j.enzmictec.2020.109584
A. Rotter, A. Bacu, M. Barbier, F. Bertoni, A.M. Bones, M.L. Cancela, J. Carlsson, M.F. Carvalho, M. Cegłowska, M.C. Dalay, T. Dailianis, I. Deniz, D. Drakulovic, A. Dubnika, H. Einarsson, A. Erdoğan, O.T. Eroldoğan, D. Ezra, S. Fazi, R.J. FitzGerald, L.M. Gargan, S.P. Gaudêncio, N.I. DeNardis, D. Joksimovic, M. Kataržytė, J. Kotta, M. Mandalakis, I. Matijošytė, H. Mazur-Marzec, A. Massa-Gallucci, M. Mehiri, S.L. Nielsen, L. Novoveská, D. Overlingė, M.E. Portman, K. Pyrc, C. Rebours, T. Reinsch, F. Reyes, B. Rinkevich, J. Robbens, V. Rudovica, J. Sabotič, I. Safarik, S. Talve, D. Tasdemir, X.T. Schneider, O.P. Thomas, A. Toruńska-Sitarz, G.C. Varese, M.I. Vasquez. A new network for the advancement of marine biotechnology in Europe and beyond. Front. Mar. Sci. 2020, 7. doi.org/10.3389/fmars.2020.00278
V. Malūnavičius, G. Druteika, M.Sadauskas, A. Veteikytė, I. Matijošytė, E. Lastauskienė, A. Gegeckas, R. Gudiukaitė. Usage of GD-95 and GD-66 lipases as fusion partners leading to improved chemiric enzyme LipGD95-GD66. Int. J. Biol. Macromol. 2018, 15 (118), 1594. doi.org/10.1016/j.ijbiomac.2018.07.002
M. Šulcienė, B. Kolvenbach, E. Ammann, I. Matijošytė. Towards an affordable enzymatic production of biopolyols – Comparing the immobilization of lipases by two optimized techniques. Int. J. Biol. Macromol. 2018, 116, 1049. doi.org/10.1016/j.ijbiomac.2018.05.046
A. Veteikytė, R. Šiekštelė, B. Tvaska, I. Matijošytė. Sequential application of waste whey as a medium component for Klyuveromyces lactis cultivation and a co-feeder for lipase immobilization by CLEA method. Appl. Microbiol. Biotechnol. 2017, 101, 3617. doi.org/10.1007/s00253-017-8131-x
V. Melvydas, I. Bruzauskaitė, G. Gedminienė, R. Šiekštelė. A novel Saccharomyces cerevisiae killer strain secreting the X factor related to killer activity and inhibition of S. cerevisiae K1, K2 and K28 killer toxins. Indian J. Microbiol. 2016, 56, 335. doi.org/10.1007/s12088-016-0589-1
R. Gruškienė, V. Kairys, I. Matijošytė. CLEA-based immobilization of methylotropic yeast alcohol oxidase: Influence on storage stability and reaction efficiency. Org. Process Res. Dev. 2015, 19 (12), 2025. doi.org/10.1021/acs.oprd.5b00291R
R. Šiekštelė, A. Veteikytė, B. Tvaska, I. Matijošytė. Yeast Kluyveromyces lactis as host for expression of the bacterial lipase: Cloning and adaptation of the new lipase gene from Serratia sp. J. Ind. Microbiol. Biotechnol. 2015, 42 (10), 1309. doi.org/10.1007/s10295-015-1655-0
M. Šulcienė, A. Karalius, I. Matijošytė. Chemo-enzymatic route for the production of biopolyol from rapeseed oil. Curr. Org. Chem. 2014, 18 (23), 3037. doi.org/10.2174/138527281823141212105146
E. Kleinaitė, V. Jaška, B. Tvaska, I. Matijošytė. A cleaner approach for biolubricant production using biodiesel as a starting material. J. Clean Prod. 2014, 75, 40. doi.org/10.1016/j.jclepro.2014.03.077
A. Veteikytė, M. Aštrauskaitė, R. Gruškienė, R. Tekorienė, I. Matijošytė. Secondary alcohol oxidase activity identified in genus of Pseudomonas isolated from the oil polluted soil. Biocat. Agricult. Biotechnol. 2013, 2 (2), 89. doi.org/10.1016/j.bcab.2012.11.005
R. Gruškienė, R. Deveikytė, R. Makuška. Quaternization of chitosan and partial destruction of the quaternized derivatives making them suitable for electrospinning. Chemija 2013, 24 (4), 325. doi.org/10.6001/chemija.2013.24.4.10
M. Meizeraitytė, R. Gruškienė, R. Makuška, G. Dienys. Modified chitosan as a spacer for protein immobilization. Biocatal. Biotransformation 2013, 31 (4), 181. doi.org/10.3109/10242422.2013.814645
S.A. Tromp, I. Matijošytė, R.A. Sheldon, I.W.C.E. Arends, M.T. Kreutzer, J.A. Moulijn, S. de Vries. Mechanism of laccase/TEMPO oxidation of benzyl alcohol. ChemCatChem 2010, 2(7), 827. doi.org/10.1002/cctc.201000068
I. Matijošytė, I.W.C.E. Arends, S. de Vries, R.A. Sheldon. Preparation and use of cross-linked aggregates (CLEAs) of laccases. J. Mol. Catal. B: Enzym. 2010, 62 (2), 142. doi.org/10.1016/j.molcatb.2009.09.019
I. Matijošytė, I.W.C.E. Arends, R.A. Sheldon, S. de Vries. Pre-steady state kinetic studies on the microsecond time scale of the laccase from Trametes versicolor. Inorg. Chim. Acta 2008, 361(4), 1202. doi.org/10.1016/j.ica.2007.05.029
S. de Vries, I. Matijošytė, R.A. Sheldon, I.W.C.E. Arends, INOR 455-Pre-steady state kinetic studies (on microsecond time scale) of the laccase from Trametes versicolor. Abstracts of Papers of the American Chemical Society, 2007, 233: 333