Short Description: The Femto Pulse system provides researchers with a powerful and automated pulsed-field capillary electrophoresis system. A meticulously designed optical detection platform and pulsed-field power enable unprecedented sensitivity, detecting nucleic acids into the lower femtogram range.
Produser: Agilent
Equipment Information:
Main characteristics:
- Separate high molecular weight DNA smears and fragments through 165 kb in about 1.5 hours.
- Detect nucleic acids down to 50 fg/µL, achieving 10 times higher sensitivity for nucleic acid smears and up to 100 times higher for nucleic acid fragments.
- Replace pulsed-field gel electrophoresis with the automated capillary electrophoresis to speed up long-read NGS library preparation and BAC analysis.
- Minimize sample degradation with fewer pipetting steps and stable reagents.
- Hold up to three, 96-well sample trays at once, allowing to program the unattended separation of up to 288 samples.
- Use only 2 µL of precious sample and achieve superior detection with unparalleled sensitivity.
Applications:
- High molecular DNA quantification
- High molecular DNA qualification
- Long-read sequencing libraries QC
- Sheared HMW DNA QC
Technical Specification: Quick Guide for the Femto Pulse
Software: Agilent ProSize Data Analysis Software
VU ID:
Location: BVI 208
Core Facility: Sequencing and Bioinformatics
Equipment is operated by Personnel only: Yes
It is allowed to work with the equipment independently after familiarization with the work process on site: No
Only those with the appropriate certificate/certificate are allowed to work with the equipment: Yes
There is an opportunity to participate in equipment usage training: Yes
Contact Scientist (Leading Scientist):
Dr. Giancarli Russo