Short Description: IVIS Lumina X5 Imaging System is a high-throughput in vivo imaging platform combining sensitive bioluminescence, fluorescence, and high-resolution X-ray imaging. It is used for non-invasive monitoring of disease progression, gene expression, and drug response in small animal research.

Produser: Revvity IVIS Lumina X5 Imaging System, Cat. No. CLS148590   Revvity RAS-4 Rodent Anesthesia System, Cat. No. CLS146737

Equipment Information: 

• High-throughput 2D bioluminescence, fluorescence, and X-ray imaging for non-invasive in vivo studies of small animals.
• Large 20 × 20 cm field of view enabling simultaneous imaging of up to 5 mice.
• Cooled CCD camera for high-sensitivity detection of low-light biological signals.
• Multispectral imaging with spectral unmixing (CPS) for separating signals and quantifying multiple fluorophores.
• 26 excitation/emission filters covering visible to near-infrared range for flexible fluorescence imaging.
• Integrated microfocus X-ray with optical overlay for combined anatomical and molecular imaging.
• Living Image software for acquisition, quantification, ROI analysis, and data management.
• Compatible with RAS-4 anaesthesia system for controlled isoflurane delivery and safe gas scavenging during imaging.

Operating Principle:

• Highly sensitive cooled CCD detection of bioluminescent and fluorescent signals emitted from labelled cells or tissues, combined with multispectral optical filtering and optional X-ray imaging, enabling non-invasive visualisation and quantification of biological processes in live animals.

Technical Specification: 

• Back-illuminated, thermoelectrically cooled Grade 1 CCD camera (2048 × 2048 pixels, 13.5 µm pixel size, -90 °C) for high-sensitivity low-light detection.
• Quantum efficiency >85% (500 nm) and >80% (650 nm) with very low noise (2e⁻ RMS) and dark current 21 lp/mm resolution) with automated optical/X-ray overlay and ~10 s acquisition time.
• Heated motorized stage (20-40 °C) with automated positioning and filter control for stable in vivo imaging conditions.
• Extended NIR illumination source (150 W tungsten) for efficient excitation and deep tissue imaging.
• Light-tight imaging chamber (37 × 37 × 43 cm) with radiation shielding and low leakage (0.1 mR/h) for safe operation

VU ID: 8152243
Location: GMC V019
Core Facility: Eukaryotic Cell Engineering

Equipment is operated by Personnel only: Yes
It is allowed to work with the equipment independently after familiarization with the work process on site: No
Only those with the appropriate certificate/certificate are allowed to work with the equipment: Yes
There is an opportunity to participate in equipment usage training: No

Contact Scientist (Leading Scientist):Indrė Dalgėdienė
Contact email address: 

Information for the User:

• As the instrument is intended for in vivo imaging of live animals, the following are mandatory: (1) appropriate personnel authorisation (e.g., certification such as FELASA Category C), and (2) formally approved protocols/permits for the planned animal experiments.
• IVIS Lumina X5 - High-sensitivity 2D optical meets High-resolution x-ray
• Modalities combined. Intelligence driven
• Anesthesia system for in vivo imaging platforms