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Equipment in Bioengineering Core:

Short Description: Varioskan LUX is a modern multifunctional microplate reader designed for absorbance, fluorescence, and luminescence measurements. The instrument supports a wide range of experimental conditions and enables both endpoint and kinetic assays, making it suitable for biochemical, cell biology, and pharmaceutical research.

Masiu fotometras 2MPProduser: Thermofisher

Equipment Information: 

Functions:

  • Measures absorbance, fluorescence, and luminescence signals from samples in microplates
  • Supports time-resolved fluorescence (TRF) and other advanced detection modes
  • Enables both endpoint and kinetic measurements
  • Allows automatic wavelength selection using monochromators
  • Offers temperature control, shaking, and optional automated dispensing

Capabilities:

  • High sensitivity detection suitable for low-signal applications
  • Broad wavelength range for UV/VIS and fluorescence studies
  • Compatible with multiple plate formats (e.g., 96- and 384-well)
  • Controlled and analyzed via SkanIt™ software
  • Expandable workflow automation options

Usage:

Varioskan LUX is widely used in:

  • Biochemical research
  • Protein quantification (BCA, Bradford, Lowry)
  • Enzyme assays and reaction monitoring
  • Cell biology
  • Cell viability, proliferation, apoptosis assays
  • Pharmaceutical development
  • High-throughput screening
  • Drug efficacy and mechanism studies
  • Immunoassays
  • ELISA, AlphaScreen, and related applications
  • Gene expression analysis
  • Fluorescent and luminescent reporter assays
  • Operating Principle
  • The sample in each microplate well is illuminated by a controlled light source.
  • Monochromators select the exact wavelength for excitation or detection.
  • Depending on the assay:
  • The instrument measures light absorbed,
  • Fluorescence emitted, or
  • Luminescent signal produced by the sample.
  • The detected optical signal is converted into an electrical output, recorded, and processed by the SkanIt™ software, which provides data visualization and quantitative analysis. 

Technical Specification: Varioskan LUX

Software:

SkanIt™ software

VU ID: 8154144
Location: BVI 116
Core Facility: Bioengineering

Equipment is operated by Personnel only: Yes 
It is allowed to work with the equipment independently after familiarization with the work process on site: No
Only those with the appropriate certificate/certificate are allowed to work with the equipment: Yes 
There is an opportunity to participate in equipment usage training: No

Contact Scientist (Leading Scientist):
Dr. Arūnas Šilanskas,
El.paštas: 
Tel.: +370 600 09405

Information for the User:

 

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