Modern Enzymology

Course unit

title

Scientific direction

Scientific code

Faculty

Department (s)

Modern Enzymology

Chemical engineering T 005

Life Sciences Center

Institute of Biotechnology

Mode of studies

Number of credits

Mode of studies

Number of credits

Lectures

0

Consultations

2

Self-studies

6

Seminars

2

Aims of course

Main purpose is to train a profitient in enzymology and to develop of subject competences. Also, to develop general competences: skills to analyze, compare, critically evaluate information, to prepare presentation for public in order to train oral presentation and communication skills.

Main topics

The scope of enzymology has expanded rapidly over the last century, from an early focus on the chemical and catalytic mechanisms of individual enzymes to more recent efforts to understand enzyme action in the context of dynamic, functional biological systems consisting of many interacting enzymes and proteins.

Main topics:

• Enzyme structures and modification of these structures;

• Specificity and functionality of enzymes;

• Enzyme stability and stabilization studies;

• Enzyme kinetics and recent enzyme kinetics studies;

• Enzymes in non-conventional media;

• Biocatalysis;

• Immobilization of enzymes;

• Enzymology in systems biology, chemical biology, bioorganic chemistry, etc.

• Enzymes - in medicine, industry and the environment.

Main literature

1)     A. Fersht „Structure and mechanism in protein science“, W.H. Freeman and company. 2002, ISBN 0-7167-3268-8.

2)     T.D.H. Bugg „Introduction to Enzyme and Coenzyme Chemistry“, Wiley, 201233rd edition, ISBN-10: 1119995949.

3)     J. Kelly „Anvances in Enzymology“, Calisto Reference, 2020, ISBN-10:1641162546.

4)     G. Williams, M. Hall “Modern Biocatalysis: Advances towards synthetic biological systems” The Royal Society of Chemistry 2018, ISBN: 978-1-78262-726-5

5)     V. Benival, A.K. Sharma” Industrial enzymes: Trends, scope and relevance”, ISBN: 978-1-63321-338-8

6)     H.Thatoi, S.Mohapatra, S.K. Das “Bioprospecting of Enzymes in Industry, Healthcare and Sustainable Environment”, Springer Narture Singapore Pte. Ltd, 2021, ISBN 978-981-334-194-4

7)     J.G. Zalatan ir D. Herschlag „The far reaches of enzymology“, Nature Chemical Biology, 2009, 5 (8) 516-520.

8)     8. C.Kettner ir M.G. Hicks „The dilema of modern functional enzymology“, Current Enzyme Inhibition, 2005, 1. 3-10

9)     A. G. Marangoni „Enzyme Kinetics: A Modern Approach“, Wiley, ISBN: 9780471159858

Assessment strategy

Assessment criteria

Seminar presentation

Seminar of 30-40 min duration. The presentation will be evaluated based on the clear visual presentation, understanding of the presented subject, relation to the leaned topics, answering to the given questions. The total score range will be from 2 to 10: 

-2-4 (insufficient)

- 5 (sufficient)

-6 (satisfactory)

- 7(highly satisfactory)

-8 (good)

-9 (very good)

-10 (excellent)

Inga Matijošytė

PhD

Marius Dagys

PhD

Approved by the Council of Graduate School of Life Sciences Center No 600000-…-… on the …. of …… 2021

Chairman