DOCTORAL COURSE UNIT DESCRIPTION
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Course unit title |
Scientific direction Scientific code |
Faculty |
Department (s) |
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Protein Interactions |
Biophysics N011, Chemical engineering T 005 |
Life Sciences Center |
Institute of Biotechnology |
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Mode of studies |
Number of credits |
Mode of studies |
Number of credits |
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Lectures |
0 |
Consultations |
2 |
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Self-studies |
8 |
Seminars |
0 |
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Aims of course |
The main goal of the course is to learn molecular interaction processes where proteins play the most important role. Attention is focused on the non-covalent, weak interaction between proteins or between proteins and small molecular weight chemical compounds that can be pharmaceuticals. |
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Main topics |
Biothermodynamics of interactions, association constant, Gibbs energy, enthalpy, entropy. Relation between protein interactions, stability and folding. Methods determining intermolecular interactions: thermal shift, izothermal titration calorimetry, inhibition of enzymatic activity, surface plasmon resonance, differential scanning calorimetry, protein X-ray crystallography and other. Biophysical graphical modeling of experimental observations. Correlations between protein structures and interactions. |
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Main literature |
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K. Dill, R. L. Jernigan, I. Bahar. 2017. „Protein Actions“. Garland Science. J. Kuriyan, B. Konforti, D. Wemmer. 2013. „Molecules of Life: Physical and Chemical Principles“. Garland Science. D. Matulis, ed. 2019. „Carbonic Anhydrase as Drug Target“. Springer-Nature. Scientific manuscripts in the field of biothermodynamics. |
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Assessment strategy |
Assessment criteria |
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Exam or seminar |
Exam consists of 5-10 questions, seminar 45 minute long. |
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Coordinator(s): Name, surname |
Scientific degree |
Pedagogical rank |
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Daumantas Matulis |
Dr. |
Prof. |
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Approved by the Council of Graduate School of Life Sceinces Center No 600000-…-… on the …. of …… 2021 |
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Chairman |