Proteomics

Course unit title

Scientific direction

Scientific code

Faculty

Department (s)

Proteomics

Biochemistry N 004

Life Sciences Center

Institute of Biochemistry

Total number of credits

Mode of studies

Number of credits

Mode of studies

Number of credits

Lectures

0

Consultations

1

Self-studies

7

Seminars

0

Aims of course

Deepening of doctoral students' knowledge about proteomics methods and technologies, the use and application possibilities of proteomics technologies in various fields of research (biomedicine, pharmacy, microbiology, agriculture, environmental science, biotechnology, etc.).

Main topics

Types of proteomics (structural, functional, expression proteomics), proteome determination methodologies, protein isolation from cell cultures and tissues and their fractionation, organelle proteomics, protein visualization and imaging, steps for protein preparation in mass spectrometry, ionization methods in proteomics, data analysis in proteomics, application of proteome research.

Main literature

• Navakauskienė R. 2011. Proteomika ir jos metodai: mokomoji knyga. Vilnius: Technika. 92 p. ISBN: 978-9955-28-885-5.
• Navakauskienė R., D. Navakauskas, V. Borutinskaitė, D.Matuzevičius. Epigenetics and Proteomics of Leukemia – A Synergy of Experimental Biology and Computational Informatics. Published by Springer, 2021, 406 pages, ISBN: 978-3-030-68707-6. https://doi.org/10.1007/978-3-030-68708-3.
• Mishra, Nawin C.; Blobel, Günter; et al. Introduction to Proteomics : Principles and Applications. John Wiley & Sons, Incorporated  2010 ISBN: ISBN number:9780471754022, ISBN number:9780470603864 (https://ebookcentral.proquest.com/lib/viluniv-ebooks/detail.action?docID=510194&query=Proteomics)
• Fuentes, Manuel; LaBaer, Joshua  Caister. Proteomics: Targeted Technology, Innovations and Applications. Academic Press  2014 ISBN: ISBN number:9781908230461, ISBN number:9781908230621 (https://ebookcentral.proquest.com/lib/viluniv-ebooks/detail.action?docID=5897822&query=Proteomics )
• Simpson, R. J. 2002. Proteins and proteomics: a laboratory manual. Cold Spring Harbor (N.Y.): Cold Spring Harbor. Laboratory Press. 926 p. ISBN 0-87969-553-6.
• International scientific review articles.

Assessment strategy

Assessment criteria

Oral exam or seminar presentation

Oral exam - 3 open questions.

Seminar presentation - duration 30-45 min.

Criteria for assessment: acquisition of knowledge and its transfer, based on the examples, apply the analysed proteomics methods to the solution of the analysed problems in the field. Consistently and reasonably explaining the details of proteomics methods and the specifics of their application for solving non-typical problems. Substantiate the suitability of the proteomics methods for solving various problems in the field of natural sciences, to freely apply the knowledge of proteomics even in unexplored, new situations, to demonstrate knowledge and skills in analysing and providing insights.

Rūta Navakauskienė

Prof.

Approved by the Council of Doctoral School of Life Sciences Center No (4.10)600000-KT-… on the 14th of October 2021

Chairman dr. Daiva Baltriukienė