Protein and cell biotechnology

Course unit

title

Scientific direction

Scientific code

Faculty

Department (s)

Protein and cell biotechnology

Biochemistry N 004

Life Sciences Center

Institute of Biochemistry

8

Mode of studies

Number of credits

Mode of studies

Number of credits

Lectures

0

Consultations

0,5

Self-studies

7,5

Seminars

0

Aims of course

To master the principles of modern biotechnology.

Main topics

• Construction of metabolic pathways in prokaryotes and lower eukaryotes by genetic engineering.
• Peculiarities of construction of plant metabolism pathways with the help of genetic engineering.
• Construction of metabolic pathways using genetic engineering in animals and the humans. Gene therapy.
• Modification of antibiotic biosynthetic pathways. 
• Production of hybrid antibiotics using in their synthesis involved modified enzymes. 
• Protein engineering. Principles of  protein construction in vitro. Development of new proteins, undetectable in nature. Purposeful change of the specificity, thermostability and other properties of enzymes.
• Modeling, engineering and production of antibodies with catalytic properties - abzymes.
• Development of protein- and cell-based vaccines.
• DNA vaccines. Principles of development and use. Prospects for clinical use.
• Methods for combinatorial polypeptide synthesis. Use for the production of recombinant proteins.
• Purposeful construction and application of multifunctional protein complexes. 
• Use of enzymes in organic chemistry. 
• Stereoselective synthesis. 
• Nanotechnologies based on biological objects and their application.
• Construction and use of transgenic organisms.

Main literature

• E. Smith Biotechnology (5th edition). Cambridge University Press, 2009
• E. Heinzle, A. Biwer and C. Cooney Development of Sustainable Bioprocesses. John Wiley & Sons, Ltd. 2006.
• A. Illanes (Editor). Enzyme Biocatalysis. Principles and Applications. Springer Science + Business Media B.V. 2008.
• G. Walsh Pharmaceutical Biotechnology. Concepts and Applications. John Wiley & Sons Ltd 2007.
• Enzyme Catalysis in Organic Synthesis, 3rd Edition. Edited by K. Drauz, H. Groger, and O.May.VCH Verlag GmbH & Co. KGaA, 2012.
• Trends in Biotechnology; Current Opinion in Biotechnology, Biotechnology and Bioengineering.

Assessment strategy

Assessment criteria

Rolandas Meškys

Approved by the Council of Doctoral School of Life Sciences Center No (4.10)600000-KT-… on the 14th of October 2021

Chairman dr. Daiva Baltriukienė