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Equipment in Sequencing and Bioinformatics Core:

Short Description: The M220 Focused-ultrasonicator offers the “Scientist’s Standard” in a compact, easy-to-use system making it the ideal DNA shearing solution for MiSeq and PGM users. AFA® technology in the M220 eliminates operator-induced variations, improves performance, increases efficiency, and provides standardized results.

Oxford Nanopore GRIDIonProduser: Covaris - Perkin Elmer

Equipment Information:

Main characteristics:

  • Controlled energy delivery
  • Isothermal processing
  • Non-contact, closed vessel
  • Quiet operation
  • User-selectable fragment lengths
  • No heat damage, high sample recovery
  • The mechanical shearing method of choice
  • Robust and reliable design

Applications:

  • Cell lysis: mammalian, bacterial, yeast
  • cfDNA Extraction from Plasma
  • DNA Extraction from Dried Blood Spots
  • DNA/RNA/Chromatin Shearing
  • DNA/RNA/Total NA/Protein Extraction from FFPE
  • Tissue lysis and extraction

 

VU ID: 8153145
Location: BVI 228
Core Facility: Sequencing and Bioinformatics

Equipment is operated by Personnel only: No
It is allowed to work with the equipment independently after familiarization with the work process on site: Yes
Only those with the appropriate certificate/certificate are allowed to work with the equipment: No
There is an opportunity to participate in equipment usage training: Yes

Contact Scientist (Leading Scientist):
Dr. Giancarli Russo

 

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