Short Description: Countess 3 FL Automated Cell Counter is an instrument for automated cell counting and analysis using brightfield and fluorescence imaging with three-channel flexibility (brightfield, two fluorescence channels). It enables quantification of live/dead cells and fluorescently labelled populations in cell culture, immunology, and biotechnology labs.
Produser: Thermofisher
Equipment Information:
Functions:
- Automated cell counting using brightfield and fluorescence imaging.
- Three-channel capability: brightfield plus two fluorescence channels (GFP, RFP).
- Measures total cell concentration (cells/mL) in suspension samples.
- Determines viability using Trypan Blue or fluorescent viability dyes.
- On-board touchscreen interface with integrated image analysis software.
- Stores images and results; data export via USB.
- Uses disposable Countess chamber slides to reduce contamination risk.
- Rapid analysis time (typically under 10 seconds per sample).
- Reduces operator variability compared to manual counting.
- Suitable for mammalian cell lines, primary cells, stem cells, and some insect cells.
- Applicable in immunology, cell biology, gene expression, and bioprocess labs.
- Supports monitoring before transfection, passaging, or cryopreservation.
Operating Principle:
- Digital microscopy combined with fluorescence excitation/emission optics and image-processing algorithms to identify, count, and classify cells based on morphology and fluorescence intensity.
Technical Specification:
- Light cube Cat. No. Excitation, nm Emission, nm Common compatible dyes / fluorescent proteins
- GFP 2.0 AMEP4951 470/22 525/50 GFP, Alexa Fluor 488, SYBR Green, FITC, Calcein, Fluo-4, MitoTracker Green, pHrodo Green
- RFP 2.0 AMEP4952 531/40 593/40 RFP, Alexa Fluor 546, Alexa Fluor 555, PE, dTomato, MitoTracker Orange, Rhodamine Red, SYTOX Orange
VU ID: 8151247
Location: BVI 121
Core Facility: Eukaryotic Cell Engineering
Equipment is operated by Personnel only: No
It is allowed to work with the equipment independently after familiarization with the work process on site: Yes
Only those with the appropriate certificate/certificate are allowed to work with the equipment: No
There is an opportunity to participate in equipment usage training: No
Contact Scientist (Leading Scientist):Indrė Dalgėdienė
Contact email address:
Information for the User: