The DNA Sequencing Center (SC), part of the Institute of Biotechnology (IBT) at the Life Sciences Center of Vilnius University, has been successfully running since March 27, 2003. The SC was founded to help researchers, both at IBT as well as other institutions in Lithuania, to process DNA samples in an efficient and economical manner. The Center is equipped with the Applied Biosystems 3130xl Genetic Analyser 16-capillary automated DNA sequencer that yields 700 to 1000 bases per template. It performs cycle sequencing reactions using fluorescent dye terminators ABI Big Dye® Terminator v3.1 on any kind of DNA (plasmid, phage or PCR product) provided by the users. We also run reactions made by the users’ themselves. Usually, the turnaround time takes 2–3 days after the receiving of samples. The sequencing of the larger samples may take longer. The results of the DNA sequencing are provided to the customer with an e-mail as a text document (.seq) and with the chromatograms provided in ABI format (.ab1).