Investigation of bioelectrochemical properties of biomolecules
Investigation of electron transport in biomolecules
Creation of biosensors and bioreactors
Investigation of the mechanism of action of biomolecules and cells in heterogeneous systems and mathematical modeling.
Whole-cell biosensors, bacterial self-organization, biofilms.
RESEARCH OWEREVIEW 2012-2017
Specially prepared carbon materials can possess direct electrochemical communication with redox centers of some redox proteins. For this reason a few types of graphite nanoparticles have been manufactured by oxidation of graphite using different protocols and characterized by AFM, Raman spectroscopy, TGA and BET analysis. Also thermally reduced graphene oxides and nanocomposite materials have been prepared and studied. Different oxidoreductases were applied and demonstrated direct electron transport between surface of the carbon materials and active center of the enzyme. These structures were applied in different technological structures. One of them - creation of mediatorless bio-anodes and bio-cathodes for bio-solar cells, bio-batteries and bio-fuel cells.
Second direction -the same structures were applied for the creation of new type of biosensors for the determination of the sugars and alcohols. Carbon materials were applied for the electrochemical monitoring of urease activity and electrochemical biosensors for urea were developed. These biosensors were applied in medicine, environment control, and for the quality control in food industry. In this field the project „Non- invasive approach to early diagnosis and prognosis of acute severe pancreatitis (AP)” was recently funded. The ambitiousness of the project is to diminish lack of information causing AP diagnostic, prognostic and followed treatment problems by deep understanding of AP biochemical processes that could help to create new diagnostic tools. Beekeeping products conserved with honey were analyzed. Monitoring of the changes of parameters of the mixtures via different storage conditions revealed a stabilizing influence of honey and additives on anti-oxidative properties, composition of flavonoids and activity of the enzymes of the mixtures.
Third direction - the same bio-electro-catalytic system was applied in bioreactors for the selective conversion of substrates into useful products. Conversion of N-hydroxy- compounds, alcohols and other substrates catalysed by oxidoreductases (laccases, peroxidases and quinoprotein dehydrogenases) was investigated. These systems were extended into multi-step synthesis of natural products & analogues involving sequences of enzymes conversion chains.
The numerical mathematical modelling of heterogeneous bio-electro-catalytic systems made possible to determine several structural and kinetic parameters of the sensor and bioreactor construction.
Bioluminescence imaging experiments were carried out to characterize spatiotemporal patterns of self-organization of bioluminescent E. coli and its mutants in standard microtiter plates. An analysis of the effects of mutations shows that spatiotemporal patterns formed due to migration (swimming) of cells near three phase contact line are not related to the chemotaxis system of bacteria. An analysis of experimental data and mathematical modelling of pattern formation indicate that pattern-forming active bacteria can be interpreted as self-phoretic cells dispersed in a liquid suspension.
Department participated in the international sheering of the food quality control knowledge. Food safety and Food safety assessment training courses were translated into Lithuanian language and distributed among food producers. Department participated in the introduction of ES Food safety legislation into food industry system in Turkey.
1. Baronas, R., Kulys, J. and Petkevičius, L., 2019. Computational modeling of batch stirred tank reactor based on spherical catalyst particles. Journal of Mathematical Chemistry, 57(1), pp.327-342. IF 1.81
2. Gineitytė, J., Meškys, R., Dagys, M. and Ratautas, D., 2019. Highly efficient direct electron transfer bioanode containing glucose dehydrogenase operating in human blood. Journal of Power Sources, 441, p.227163. IF 7.467
3. Gružauskaitė, J., Jasinskaitė, J., Meškys, R., Gaidamavičienė, G., Žalga, A., Laurynėnas, A., Tetianec, L. and Dagys, M., 2019. Gold-coated magnetic nanocatalyst containing wired oxidoreductases for mediatorless catalysis of carbohydrate oxidation by oxygen. Catalysis Communications, p.105848. IF 3.674
4. Laurinavičius, V., Ivanauskas, F. and Nečiporenko, A., 2019. Drug delivery mathematical modeling for pressure controlled bioreactor. Journal of Mathematical Chemistry, 57(8), pp.1973-1982. IF 1.81
5. Laurynėnas, A., Butkevičius, M., Dagys, M., Shleev, S. and Kulys, J., 2019. Consecutive Marcus electron and proton transfer in Heme peroxidase compound ii-catalysed oxidation Revealed by Arrhenius plots. Scientific reports, 9(1), pp.1-11. IF 4.011
6. LEDAS, Ž., ŠIMKUS, R. and Baronas, R., 2019. COMPUTATIONAL MODELING OF SELF-ORGANIZATION OF BACTERIAL POPULATION CONSISTING OF SUBPOPULATIONS OF ACTIVE AND PASSIVE CELLS. Journal of Biological Systems, 27(03), pp.365-381. IF 0.839
7. Makaras, T., Razumienė, J., Gurevičienė, V., Šakinytė, I., Stankevičiūtė, M. and Kazlauskienė, N., 2020. A new approach of stress evaluation in fish using β-d-Glucose measurement in fish holding-water. Ecological Indicators, 109, p.105829. IF 4.49
8. Ramonas, E., Ratautas, D., Dagys, M., Meškys, R. and Kulys, J., 2019. Highly sensitive amperometric biosensor based on alcohol dehydrogenase for determination of glycerol in human urine. Talanta, 200, pp.333-339. IF 4.916
Ratautas, D., Ramonas, E., Marcinkevičienė, L., Meškys, R. and Kulys, J., 2018. Wiring Gold Nanoparticles and Redox Enzymes: A Self‐Sufficient Nanocatalyst for the Direct Oxidation of Carbohydrates with Molecular Oxygen. ChemCatChem, 10(5), pp.971-974.
Gruskiene, R., Krivorotova, T., Staneviciene, R., Ratautas, D., Serviene, E. and Sereikaite, J., 2018. Preparation and characterization of iron oxide magnetic nanoparticles functionalized by nisin. Colloids and Surfaces B: Biointerfaces, 169, pp.126-134.
Baronas, R., Kulys, J. and Petkevicius, L., 2018. Modelling the enzyme catalysed substrate conversion in a microbioreactor acting in continuous flow mode. NONLINEAR ANALYSIS-MODELLING AND CONTROL, 23(3), pp.437-456.
Dagys M., Laurynėnas A., Ratautas D., Kulys J., Vidžiūnaitė R., Talaikis M., Niaura G., Marcinkevičienė L., Meškys R., Shleev S. Oxygen electroreduction catalysed by laccase wired to gold nanoparticles via the trinuclear copper cluster. Energy & Environmental Science , 2017, Vol. 10, p. 498-502.
Tetianec L., Chaleckaja A., Kulys J., Janciene R., Marcinkeviciene L., Meskiene R., Stankeviciute J., Meskys R. Characterization of methylated azopyridine as a potential electron transfer mediator for electroenzymatic systems. Process Biochemistry. 2017, Vol. 54, p. 41-48.
Ratautas D.,Tetianec L., Marcinkevičienė L., Meškys R., Kulys J. Characterization of methylated azopyridine as a potential electron transfer mediator for electroenzymatic systems. Biosensors Bioelectronics. 2017, Vol. 98, p. 215-221
Gaidukevic J., Razumiene J., Sakinyte I., Rebelo S. L.H., Barkauskas J.. Study on the structure and electrocatalytic activity of graphene-based nanocomposite materials containing (SCN)n. Carbon. 2017, Vol. 118, p. 156 – 167.
Ratautas D., Laurynėnas A., Dagys M., Marcinkevičienė L., Meškys R., Kulys J. High current, low redox potential mediatorless bioanode based on gold nanoparticles and glucose dehydrogenase from Ewingella Americana. Electrochimica Acta. 2016, Vol. 199, p. 254-260.
Ivanauskas F., Katauskis P., Laurinavicius V. Impact of convective transport and inert membrane on action of bio-catalytic filtre. Journal of Mathematical Chemistry. 2016, 54, (6), p. 1221-1232.
Šimkus R., Meškienė R., Ledas Ž., Baronas R., Meškys R. Microtiter plate tests for segregation of bioluminescent bacteria. Luminescence. 2016, Vol. 31, No 1, p. 127-134.
Razumiene J., Sakinyte I., Barkauskas J., Baronas R. Nano-structured carbon materials for improved biosensing applications. Applied Surface Science. 2015, Vol. 334, p. 185-191.
Razumiene J., Cirbaite E., Razumas V., Laurinavicius V. New mediators for biosensors based on PQQ-dependent alcohol dehydrogenases. Sensors and Actuators B: Chemical. 2015, 207, p. 1019-1025.
Ratautas D., Marcinkevičienė L., Meškys R., Kulys J. Mediatorless electron transfer in glucose dehydrogenase/laccase system adsorbed on carbon nanotubes. Electrochimica Acta. 2015, Vol. 174, p. 940-944.
Tetianec L., Chaleckaja A., Vidžiūnaitė R., Kulys J., Bachmatova I., Marcinkevičienė L., Meškys R. Development of a laccase/syringaldazine system for NAD(P)H oxidation. Journal of Molecular Catalysis. B: Enzymatic. Amsterdam: Elsevier. 2014, Vol. 101, p. 28-34.
Dagys M., Lamberg P., Shleev S., Niaura G., Kulys J., Arnebrant Th., Ruzgas T., Comparison of bioelectrocatalysis at Trichaptum abietinum and Trametes hirsuta laccase modified electrodes. Electrochimica Acta. Oxford : Pergamon-Elsevier Ltd. 2014, Vol. 130, p. 141-147.
Ivanauskas F., Katauskis P., Laurinavičius V. Mathematical modeling of biosensor action in the region between diffusion and kinetic modes. Journal of Mathematical Chemistry. 2014, Vol. 52, p. 689-702.
Simelevicius D., Petrauskas K., Baronas R., Razumiene J. Computational Modelling of Mediator Oxidation by Oxygen in Amperometric Glucose Biosensor. Sensors. 2014, Vol. 14, No. 2, p. 2578-2594.
Snopok B., Naumenko D., Servienė E., Bružaitė I., Stogrin , Kulys J., Snitka V., Evanescent-field-induced Raman scattering for bio-friendly fingerprinting at sub-cellular dimension. Talanta. Amsterdam: Elsevier Science. 2014, vol. 128, p. 414-421.
Barkauskas J., Dakševič J., Budrienė S., Razumienė J., Šakinytė I. Adhesion of graphene oxide on a transparent PET substrate: a study focused on the optimization process. Journal of Adhesion Science and Technology. 2014, Vol. 28, No. 20, p. 2016-2031.
Laurinavicius V., Razumiene J., Gureviciene V. Bio-electrochemical Conversion of Urea on Carbon Black Electrode and Application. IEEE Sensors. 2013, Vol. 13, No. 6, p. 2208-2213.
Ašeris V., Baronas R., Kulys J. Modelling the biosensor utilising parallel substrates conversion. Journal of Electroanalytical Chemistry. 2012, Vol. 685, p. 63–71.
Kulys J., Bratkovskaja I., Ašeris V., Baronas R. Electrochemical Peroxidase-Catalase Clark-Type Biosensor: Computed and Experimental Response. Electroanalysis. 2013, Vol. 25, No. 6, 1491 – 1496.
National Research Projects
Research Council of Lithuania
Self-organization of E. coli and their mutants near three phase contact line. (2014-2016) Dr. R. Šimkus.
Bee products enriched with plant components, the composition and properties. (2012-2015) Dr. B. Kurtinaitiene.
Biotechnology and Biopharmacy: fundamental and applied research, activity 1.1.4. (2012-2015) Head of the Action Dr. J. Razumiene.
Non- invasive approach to early diagnosis and prognosis of acute severe pancreatitis (AP). (2017-2021) Dr. J. Razumiene.
Lithuanian Agency for Science, Innovation and Technology.
EUREKA Project E! 8835. Multiple biosensor device for hemodialysis patients. (2014-2016) Dr. J. Razumiene.
Development of carbamide analyser for measurements in industrial media (2017 – 2018) Dr. J. Razumienė
High technology development program “Search of new and genetically modified oxidoreductases for creation of biofuel cells (BIOFUELCELL), (2011-2013). Coordinator – JSC “Lithuanian Research Centre”, manager – dr. Marius Dagys.
International Research Projects
7 FW Leonardo da Vici Program. „Food Industry - Food Legislation, Impact Analysis, Training and Cooperation Network in Europe -E-Learning“ (2012-2014). Prof. V. Laurinavicius.
COST CM0701 “Cascade Chemoenzymatic Processes – New Synergies Between Chemistry and Biochemistry“ (CASCAT). (2010-2014) Dr. J. Razumiene.
Foundation of dr. Bronislovas Lubys. Applied Charity and Support.“Amperometric fast response method for measurement of urea concentration in industrial media”.(2015 – 2016) dr. Marius Dagys.
Head of department
|Jadvyga Matulevič Ilinykh