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Institute of Biochemistry

Institute of Biochemistry is a research centre focusing on investigation of biochemical and genetic principles of cell functioning - genetic engineering, biocatalysis, signaling and cell regulation, basic and applied aspects of enzyme chemistry, biosensor design, synthesis of the organic compounds.

The staff of the Institute consists of experienced, qualified and competent more than 50 scientists, 30 doctoral students  with expertise in the field of biochemistry, microbiology, cellular biology and chemistry.

The Institute of Biochemistry operates at the interface between medicine, biology and chemistry to investigate the structure and function of cells, biomolecules, (bio)materials.

Institute provides infrastructure for postgraduate studies leading to Masters and PhD qualifications.

Main R&D activities

Investigation and application of biocatalysts and self-assembling structures

  • Screening, development and application of biocatalysts
  • Metabolic pathways in microorganisms
  • Genetic diversity and molecular biology of bacteria and bacteriophages
  • Metabolism of modified nucleotides and nucleosides
  • Self-assembling lipid systems and their interactions with proteins
  • Investigation of the electron transport in proteins, the structure and functioning of enzymes
  • Development and investigation of bioelectrocatalytical systems for biosensors and bioreactors
  • Synthesis, biochemical characterization and cytotoxicity studies of redox active xenobiotics and other organic compounds
  • Search and development for methods of synthesis of bioorganic compounds as modulators of biologic processes

Signaling pathways and epigenetic regulation of tumor and stem cells

  • Studies of the molecular mechanisms of signal transduction in normal and cancer cells
  • Epigenetic regulation in cancer and stem cells
  • High-throughput proteomics analysis of cell signaling
  • Elucidation of artificial microstructures and nano-particles on cell functioning
  • Adult stem cells properties and their sensitivity in vitro and in vivo
  • Cell technologies for the regenerative medicine: development and evaluation using biological models

Research services

Screening of novel enzymes for biocatalysis

  • Biodiversity of catabolic pathways.
  • Screening and development of enzymes including metagenomic libraries and genetic engineering.
  • Application of enzymes for bioconversion and biosensors.
  • Understanding of the genetics, biochemistry and mechanisms of biodegradation of various N-heterocycles (hydroxy-, methyl- and carboxypyridines and pyrazines) and development of biocatalytic processes for conversion of glycerol, starch and other carbohydrates.

Protein analysis and their sub-subcellular localization

  • Protein identification, purity and stability analysis.
  • Peptide de novo sequencing.
  • Protein-protein complex formation (dimerization, oligomerization, etc.) analysis.
  • Identification of protein chemical modification (cell signaling).
  • Proteome differential screening (marker discovery).
  • Hypothesis-driven protein analysis (marker verification).
  • Protein co-localization in cells (study of protein function).

Cell technologies

  • Microinjection of proteins and other substances into the live cell to study their function.
  • Screening of novel chemical compounds for potential therapeutic activity.
  • Studies of cytotoxicity.
  • Cellular microenvironment modeling in vitro.
  • Construction and testing of scaffolds designed for regenerative medicine.
  • Fabrication of artificial tissues.

In vivo testing

  • Preclinical studies of novel materials and chemical compounds: acute and repeated dose toxicity tests (oral, dermal, skin irritation, eye irritation, skin sensitization).
  • Biocompatibility tests in vivo.

Biosensors design

  • Bioanalytical systems for food-quality control, medicine, environment.
  • Determination of enzymes and metabolites in food-stuffs, blood, microbiological liquids.

Synthesis of organic compounds

  • Investigation of synthesis methods for organic compounds of different classes
  • Design and development of technology of chemical processes for commerce and industry

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